FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665375
Sequences flagged as poor quality0
Sequence length6
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAATT41790.6280668795791847No Hit
GGGGGG30280.4550817208341161No Hit
GTGGGG17850.26826977268457636No Hit
TGGGGG17150.25774938944204395No Hit
GGGTGG16410.24662784144279543No Hit
GGGGTG15330.2303963930114597No Hit
AGGGGG15220.2287431899304903No Hit
GGGGCG14060.21130941198572234No Hit
GGTGGG13080.19658087544617697No Hit
CGGGGG12700.19086981025737365No Hit
GGGGTC12040.1809505917715574No Hit
GGCGGG11920.17914709750140898No Hit
AGGGGT11490.1726845763667105No Hit
GAGGGG10560.15870749577306031No Hit
GGGAGG10550.15855720458388128No Hit
ATAAGG10440.1569040015029119No Hit
GGGGGT10380.15600225436783768No Hit
GTGAGG10150.1525455570167199No Hit
GGGGAG10040.1508923539357505No Hit
GGGTTG9970.14984031561149727No Hit
GGCGAG9940.14938944204396018No Hit
GGTGTG9870.14833740371970694No Hit
TGGTGG9660.14518128874694722No Hit
GCGGGG9280.1394702235581439No Hit
GTCGAG9270.13931993236896487No Hit
ATGTGG9170.13781702047717453No Hit
TTGGGG9130.13721585572045839No Hit
TGCGGG9120.13706556453127935No Hit
TGAGGG9000.13526207026113093No Hit
GTGGGC8960.13466090550441479No Hit
GGGGGA8870.13330828480180348No Hit
GATGGG8840.1328574112342664No Hit
GGCTGG8450.12699605485628404No Hit
GGGCGG8440.12684576366710504No Hit
GTGTGG8410.12639489009956792No Hit
TGGGGT8340.12534285177531468No Hit
GCCGGG8340.12534285177531468No Hit
GAGGTG8320.1250422693969566No Hit
GTAGGA8310.12489197820777756No Hit
GTAGGG8310.12489197820777756No Hit
GGCTTG8250.12399023107270338No Hit
GTTCGG8220.12353935750516626No Hit
GAGGGT8120.1220364456133759No Hit
GGTGGA8090.12158557204583881No Hit
GAGGAC8050.12098440728912267No Hit
TTGGAA7990.12008266015404846No Hit
GGTTGA7960.11963178658651137No Hit
GAAGGG7950.11948149539733233No Hit
GCATGA7850.11797858350554198No Hit
GGGGGC7780.11692654518128875No Hit
GTTGGG7750.11647567161375164No Hit
GTTTGT7710.1158745068570355No Hit
GGCCGG7700.11572421566785648No Hit
CGGAAG7670.11527334210031936No Hit
AGTGGT7660.11512305091114032No Hit
GTGGTG7570.11377043020852902No Hit
GTCCGG7510.11286868307345482No Hit
GGGTAG7410.11136577118166448No Hit
CTGGGG7360.11061431523576931No Hit
GTCGGG7350.11046402404659027No Hit
CGGGCT7300.10971256810069509No Hit
GGTTGG7270.109261694533158No Hit
GTGGCG7230.10866052977644186No Hit
TGGAGA7220.10851023858726283No Hit
AGTGGG7200.10820965620890474No Hit
ATTGGG7180.1079090738305467No Hit
AGGTGG7180.1079090738305467No Hit
CCGGGG7140.10730790907383056No Hit
GTGGGT7120.10700732669547247No Hit
GGGGCC7110.10685703550629344No Hit
TGGGTG7090.10655645312793538No Hit
ATGGGT7040.1058049971820402No Hit
ATGGCG6950.1044523764794289No Hit
GGTCGG6940.10430208529024985No Hit
TGGTGT6870.10325004696599661No Hit
AATGGG6860.1030997557768176No Hit
GGAGGG6850.10294946458763855No Hit
GTGTCG6830.10264888220928048No Hit
TGTTGG6830.10264888220928048No Hit
GGCTGA6770.10174713507420628No Hit
CGGGGT6730.10114597031749015No Hit
TGGAGG6730.10114597031749015No Hit
GGGATA6690.100544805560774No Hit
GAGTGG6690.100544805560774No Hit
GGAGCG6670.10024422318241594No Hit
GTTGGT6670.10024422318241594No Hit
GCGGCG6660.10009393199323689No Hit
AGGGTT6660.10009393199323689No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)