FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13763883
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3213782.3349370232222983Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1454700.0131.291821
CTGCAAT1451200.0131.096682
ACCGCAA205100.0125.824211
TGCAATT1735250.0124.531233
CCGCAAT207450.0124.185242
CGCAATT226550.0123.218813
GCAATTT2364150.0103.8998644
AATTTCC2416450.0102.10546
CAATTTC2415350.0101.901555
TTTCCAC2430000.0100.5727848
TTCCACA2462150.099.497349
ATTTCCA2479450.099.143017
CGGCAAT37650.070.486242
ATGCAAT256800.059.7177732
ACGGCAA48450.054.837491
TATGCAA92950.054.1220361
ATTGCAA123850.053.4550971
ACGCAAT31500.046.2672582
TCGCAAT18900.046.037072
GGCAATT64650.044.188383
Produced by FastQC (version 0.11.5)