FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11183825
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT10433629.329205347901992Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA124000.11087441014143193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1585950.0135.974271
CTGCAAT1580100.0135.760062
CCGCAAT216250.0130.87062
TGCAATT1882400.0128.259753
ACCGCAA222800.0127.580921
CGCAATT240950.0127.020133
GCAATTT2600650.0105.389644
AATTTCC2637550.0104.4273156
CAATTTC2631550.0104.4221655
TTTCCAC2641050.0103.414528
ATTTCCA2678650.0102.463627
TTCCACA2674550.0102.354129
CGGCAAT36200.089.7364962
ACGGCAA45900.071.796191
ATTGCAA110900.067.677951
ATGCAAT230000.066.993212
GGCAATT52550.064.435963
TATGCAA73400.060.225371
CATGCAA87800.052.580031
ACGCAAT30250.050.337732
Produced by FastQC (version 0.11.5)