FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12042457
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6424075.33451769850621Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT270430.22456380786744765Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT216360.17966433261916567Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT183340.15224467897207355Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT151970.12619517761201057Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT752850.0126.283732
TGCAATT814750.0125.4932563
ACTGCAA780250.0120.3877641
CGCAATT95850.0116.740573
GCAATTT1008250.0113.781774
AATTTCC1144550.0110.573826
CAATTTC1094050.0109.522995
CCGCAAT98500.0109.472982
TTTCCAC1193000.0105.4084248
ATTTCCA1206400.0104.652517
ACCGCAA106100.0100.5673141
TTCCACA1256150.0100.49439
GACCGGA55900.074.5618742
AGACCGG56900.071.614511
CGGCAAT30300.060.391332
ACCGGAA72500.057.789523
GATTGGA102550.048.9281852
GTTCCAC92150.048.6178445
AGATTGG103850.047.5612031
ACGGAAG59800.047.355964
Produced by FastQC (version 0.11.5)