FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14580790
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5969604.094154020461168Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1357900.0132.034652
ACTGCAA1381550.0130.316791
TGCAATT1555200.0127.5861743
CGCAATT199600.0125.002463
CCGCAAT191600.0122.238752
ACCGCAA194100.0120.722511
GCAATTT2087850.0107.535584
CAATTTC2125350.0105.8053745
AATTTCC2136300.0105.7050556
TTTCCAC2158250.0103.7807548
TTCCACA2196900.0102.179699
ATTTCCA2204250.0102.072157
CGGCAAT37400.067.469812
ATGCAAT209450.060.5149882
ACGGCAA50000.049.942321
ATTGCAA107200.045.1659661
TATGCAA84350.044.062091
GGCAATT60900.043.4580273
CATGCAA102900.042.609051
ACGCAAT26100.041.6727942
Produced by FastQC (version 0.11.5)