FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15113691
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT12279918.125023860815999Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT507990.33611246915131454Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT403660.26708234275796694Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT345490.2285940608419214Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT285440.18886187364820411Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT858200.0125.034143
CTGCAAT810800.0124.536832
ACTGCAA849700.0117.7841
CGCAATT95200.0114.4233253
GCAATTT1054250.0113.230694
AATTTCC1186200.0110.9893346
CAATTTC1135300.0109.722665
TTTCCAC1239250.0105.450868
ATTTCCA1262450.0104.01247
TTCCACA1313650.099.824779
CCGCAAT105250.099.498122
ACCGCAA114950.090.250161
GACCGGA92450.085.308972
AGACCGG92350.083.544211
ACCGGAA112400.070.100913
TGATCGG33350.061.648051
GATTGGA149150.060.3768122
AGATTGG147500.059.892211
ATTGGAA162450.055.4769133
ACGGAAG90450.054.9972274
Produced by FastQC (version 0.11.5)