FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4350485
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1811274.163374888087191Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA51650.11872239531914258No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA43880.10086231764964136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA664800.0136.325911
CTGCAAT671150.0134.475022
CCGCAAT91250.0132.146592
ACCGCAA93050.0130.270521
TGCAATT782900.0125.642643
CGCAATT102000.0125.326723
GCAATTT1069100.0104.656764
AATTTCC1083450.0103.669316
CAATTTC1081000.0103.658945
TTTCCAC1079550.0103.1198
ATTTCCA1096250.0102.130097
TTCCACA1096600.0101.81329
CGGCAAT14450.087.81462
ATTGCAA39350.069.356821
GGCAATT20400.064.689343
ACGGCAA20050.064.077411
CATGCAA32800.060.4136241
ATGCAAT86950.058.87512
TCGCAAT7350.051.299432
TATGCAA24150.044.4826471
Produced by FastQC (version 0.11.5)