FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11573951
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5108834.414076057519165Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT349930.3023427349917068Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT181570.15687814817947648Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT168660.14572378956848875Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT148660.12844360581792683Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1036550.0130.834182
ACTGCAA1056200.0128.962621
TGCAATT1167300.0126.310633
ACCGCAA136050.0121.236641
CGCAATT144150.0119.8889163
CCGCAAT137850.0119.5117342
GCAATTT1494550.0111.661654
AATTTCC1644750.0110.1729356
CAATTTC1578850.0109.3464055
TTTCCAC1696250.0106.104498
ATTTCCA1703750.0105.990557
TTCCACA1785300.0101.096279
CGGCAAT36150.080.8847962
GACCGGA44600.069.474252
ACGGCAA45150.065.103531
GTTCCAC108100.062.305035
AGACCGG49850.061.738511
GATTGGA117550.059.7109222
AGATTGG118250.059.0678331
ATTGGAA131900.052.8836063
Produced by FastQC (version 0.11.5)