FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9183678
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT148897416.213264445900652Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT499610.5440195093948198Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT307460.3347896126149022Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT289860.3156251776249124Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT258000.2809331947396239Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC102410.11151305609800344Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT101200.11019550119244162Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC100200.10910661284073767RNA PCR Primer, Index 16 (96% over 25bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT99150.10796328007144851Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC97160.1057963922515576RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT706950.0132.948172
TGCAATT779150.0130.136873
ACTGCAA735100.0128.22041
CGCAATT98800.0119.971663
CCGCAAT97400.0115.05482
GCAATTT1027200.0111.858534
AATTTCC1103050.0110.018616
CAATTTC1070400.0109.804865
TTTCCAC1129850.0106.7901158
ATTTCCA1144100.0105.834417
ACCGCAA108650.0103.143991
TTCCACA1186350.0101.874219
GACCGGA57150.091.8040852
AGACCGG58600.089.841231
AGATTGG103600.084.485021
GATTGGA104600.083.203672
ATTGGAA107500.080.750733
CGGCAAT26350.076.49662
ACGGAAG61900.070.882844
GAACGGA61900.069.947032
Produced by FastQC (version 0.11.5)