FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10304327
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT7569007.345457883857917Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA161250.1564876580488954No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA133930.12997452429450268No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAT111830.10852722356346027No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA2007950.0138.441381
CTGCAAT2004600.0138.016192
CCGCAAT284400.0135.097692
ACCGCAA287050.0133.810751
CGCAATT310800.0130.175863
TGCAATT2312200.0129.94863
CGGCAAT35800.0108.7614062
GCAATTT3187650.0107.5480354
CAATTTC3210950.0106.923425
AATTTCC3217750.0106.87816
TTTCCAC3203250.0106.3824168
ATTTCCA3246100.0105.552117
TTCCACA3243950.0105.273439
ACGGCAA44900.087.1471561
GGCAATT50450.080.195553
ATTGCAA94200.074.676451
TATGCAA64400.071.2429661
ATGCAAT228350.067.8875662
CATGCAA78150.062.516821
GCAATTC80300.052.461154
Produced by FastQC (version 0.11.5)