FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8076362
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT4727065.85295706160769Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT195930.24259685239467968Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT156230.19344105675302817Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT133360.1651238515559357Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT108910.13485031998318053Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT481450.0125.875352
TGCAATT524900.0124.098643
ACTGCAA499550.0120.237841
CGCAATT64550.0113.837943
GCAATTT659450.0111.3960954
AATTTCC738450.0109.376646
CCGCAAT65150.0108.779972
CAATTTC707200.0108.657215
TTTCCAC771500.0104.1035468
ATTTCCA782450.0103.063447
ACCGCAA70900.098.9687351
TTCCACA815550.098.774599
GACCGGA38800.075.98152
AGACCGG40200.072.819241
CGGCAAT19850.063.6505622
ACCGGAA52350.056.452153
AGATTGG72200.050.6055221
GATTGGA73100.050.3621332
ACGGAAG41350.049.8704034
GTTCCAC63050.048.137465
Produced by FastQC (version 0.11.5)