FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12569719
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT136168110.833026577602888Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT939040.7470652287453681Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT484150.3851716971556803Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT442730.3522194887570677Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT405360.32248930942688536Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC139450.11094122310928352RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC139210.11075028805337653RNA PCR Primer, Index 16 (96% over 25bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT133910.1065338055687641Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT997550.0129.041322
TGCAATT1106100.0125.8066253
ACTGCAA1033300.0124.979211
CGCAATT139850.0119.723783
CCGCAAT136250.0117.709462
ACCGCAA141850.0113.044151
GCAATTT1405150.0112.680784
AATTTCC1542500.0111.438136
CAATTTC1481100.0110.417815
TTTCCAC1594750.0107.184698
ATTTCCA1608950.0106.436957
TTCCACA1686550.0101.436529
GACCGGA83050.093.53432
AGACCGG84850.092.091221
GATTGGA182950.089.451852
AGATTGG186150.088.252441
ACCGGAA96050.081.101643
AGAACGG90850.078.561521
ATTGGAA209150.077.96783
ACGGAAG92950.076.293814
Produced by FastQC (version 0.11.5)