FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9963001
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT337459533.87127031303118Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT312470.31363040112110796Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC293670.29476058468728444RNA PCR Primer, Index 16 (96% over 25bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT285760.2868212097941173Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC282730.28377995746462337RNA PCR Primer, Index 16 (96% over 25bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT261890.26286256520500195Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC216440.21724378026259356Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC176280.17693464047629825Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC157350.15793434126926215RNA PCR Primer, Index 16 (100% over 22bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT149560.15011541201290654Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC142760.143290159260247TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCCATCTCGTATGCCGTCTT138660.1391749333358493Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC128230.12870620006963765TruSeq Adapter, Index 16 (95% over 22bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA124300.12476160546405646No Hit
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC107870.10827059035726284TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACACGTCTCAACTCCAGTCCATCTCGTATGCCGTCTT106160.10655424003269698Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA104320.1047074069349185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)