FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4572055
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2540145.555794932475659Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA65990.1443333468210684No Hit
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGCAAAA53700.11745265531582627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA806400.0140.021851
CTGCAAT807950.0139.276432
CCGCAAT109600.0134.319732
ACCGCAA111100.0132.620861
CGCAATT119550.0132.112413
TGCAATT951500.0130.910323
GCAATTT1317250.0107.216624
AATTTCC1328750.0106.8204046
CAATTTC1325850.0106.6567465
TTTCCAC1334200.0105.4160848
ATTTCCA1350300.0104.755147
TTCCACA1350550.0104.349259
CGGCAAT16200.093.5590062
ATTGCAA40650.081.996621
TATGCAA35450.081.7335741
ACGGCAA19000.079.497521
GGCAATT20050.077.037213
ATGCAAT102400.073.6525342
TACGCAA5950.069.566751
CATGCAA33150.059.8029981
Produced by FastQC (version 0.11.5)