FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16182038
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT441695827.295437076590723Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1817031.1228684545172865Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1449700.8958698527342477Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1241270.7670665462533212Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1013780.6264847480892085Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC428300.26467617984829844Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT402670.24883763095847386Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT349330.21587515738128904Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT340970.21070893542580976Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT306000.18909855482974394Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC271420.167729182195716RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC265390.1640028283211299RNA PCR Primer, Index 16 (96% over 25bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC213570.13197966782675952Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC208510.12885274401160102Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT200110.12366180329078452Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT188750.11664167393501362Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGATCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT182830.11298329666510486Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGA219200.0118.133212
CTGCAAT628900.0116.596362
TGCAATT675800.0116.43353
AGACCGG222800.0114.44461
GCAATTT817200.0107.598724
GATTGGA302000.0107.048212
AGATTGG301700.0105.969151
ACTGCAA687600.0105.2903061
AATTTCC927100.0104.653516
ACCGGAA249950.0104.034743
CAATTTC889100.0103.2222065
TTTCCAC955000.0101.021028
CCGGAAG261200.098.94234
TGATCGG68950.098.720811
ATTTCCA992650.097.5982367
CGCAATT86450.096.008223
ATTGGAA340400.095.24873
TTCCACA1029150.093.944719
TTGGAAG355950.090.924474
AGAACGG191350.090.013251
Produced by FastQC (version 0.11.5)