FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483908

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483908
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11465770
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6645745.796156734349285Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT275440.24022808760336198Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT218930.19094225682182706Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT186130.1623353686669103Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT156920.13685953930699812Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT905050.0131.711332
TGCAATT985750.0129.702583
ACTGCAA942600.0124.9743351
CGCAATT117700.0123.0965353
GCAATTT1244850.0115.844154
AATTTCC1388100.0114.382566
CCGCAAT120150.0114.300752
CAATTTC1330300.0113.144625
TTTCCAC1448600.0109.183748
ATTTCCA1457500.0108.755697
ACCGCAA127200.0106.91781
TTCCACA1533800.0103.4002469
CGGCAAT33950.075.512042
GACCGGA55400.074.0661852
AGACCGG56050.071.806781
GTTCCAC96950.060.973995
TGATCGG22650.059.6590081
ACCGGAA70750.058.3043943
ACGGCAA46200.054.2510831
AGATTGG99450.052.523711
Produced by FastQC (version 0.11.5)