FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9760567
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3788803.8817417061939126Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT98740.10116215584606919Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT571850.0123.797492
TGCAATT628300.0122.475793
ACTGCAA582700.0121.4656141
CGCAATT75800.0109.991083
GCAATTT796750.0108.473014
ACCGCAA73100.0107.548621
CCGCAAT74050.0106.388192
AATTTCC862950.0105.68256
CAATTTC845800.0104.901345
TTTCCAC892450.0101.4581768
ATTTCCA910500.099.9883967
TTCCACA916550.098.8774269
CGGCAAT24250.061.6231842
GACCGGA30350.052.1058462
AGACCGG32100.048.8475151
TCCGATC32250.041.3981935
ACGGCAA35750.041.2205471
CCGATCT38500.039.366956
ATGCAAT103850.037.9299242
GGCAATT46250.035.927783
Produced by FastQC (version 0.11.5)