FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11067282
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT231732920.93855564536984Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT590590.5336359912036216Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT525380.474714568581518Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT375250.33906247261070965Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT363550.3284907712661519Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT160960.14543769644615542Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT141560.12790855062697418Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTCTGCCGTCTT137000.1237882977952491Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGACGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134260.12131253183934412Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC133250.12039993197968571RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC130950.1183217342794735Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC130030.11749045519938862RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT580450.0122.998863
CTGCAAT548500.0122.181452
ACTGCAA580250.0115.561811
GCAATTT731600.0109.447254
CGCAATT70000.0109.0406653
AATTTCC797700.0106.823916
CAATTTC777650.0106.007245
TTTCCAC824750.0102.7492458
ATTTCCA844100.0100.7889947
GACCGGA90500.099.787862
TTCCACA865300.098.1352849
AGACCGG92600.097.512641
CCGCAAT75150.097.391072
ACCGGAA108300.083.676743
GATTGGA124800.082.824192
AGATTGG124850.082.440241
ACCGCAA96350.076.239551
ATTGGAA137650.074.057823
TCGGACG31950.071.533024
ACGGAAG88800.069.3683854
Produced by FastQC (version 0.11.5)