FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9416774
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6142666.523104409217Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1101600.0133.109381
CTGCAAT1104400.0132.353012
CCGCAAT160000.0126.8515242
TGCAATT1313300.0125.553193
ACCGCAA163300.0125.0445561
CGCAATT176200.0124.156843
GCAATTT1789650.0104.9982454
AATTTCC1822200.0103.843146
CAATTTC1821200.0103.398265
TTTCCAC1827150.0102.606898
TTCCACA1849950.0101.419599
ATTTCCA1859700.0101.3339167
CGGCAAT27700.083.768932
ATGCAAT180050.067.377612
TATGCAA68050.065.8170241
ACGGCAA36500.063.64121
ATTGCAA85150.062.9148141
GGCAATT42200.057.905353
ACGCAAT25950.051.1359672
CATGCAA72050.048.158741
Produced by FastQC (version 0.11.5)