FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4758249
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT3110806.537699056943006Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT129150.2714233744387904Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103400.217306828625404Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT88110.18517315928611555Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT72680.15274526406667663Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT414650.0132.67782
TGCAATT458400.0129.139623
ACTGCAA421500.0128.988191
CGCAATT49500.0122.055713
CCGCAAT47850.0118.3729862
GCAATTT573650.0115.1445544
AATTTCC638450.0113.376616
CAATTTC608950.0112.902315
ATTTCCA659550.0109.329177
TTTCCAC656950.0109.210358
ACCGCAA52000.0108.829821
TTCCACA691450.0104.130669
GACCGGA23500.080.033512
CGGCAAT14200.075.6856162
AGACCGG25150.075.67931
GTTCCAC39800.063.3100475
ACCGGAA30200.062.037283
TGATCGG8450.060.180411
GATTGGA41600.057.779612
AGATTGG42850.054.9297941
Produced by FastQC (version 0.11.5)