FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9807549
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2981443.0399440267899758Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT206200.2102462093230429Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT104420.10646900668046623Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT843250.0129.527562
ACTGCAA853350.0128.439761
TGCAATT964050.0124.168913
CGCAATT115450.0123.480253
ACCGCAA112800.0120.926331
CCGCAAT113150.0120.1406862
GCAATTT1233300.0110.5235754
AATTTCC1352500.0109.499016
CAATTTC1296350.0108.761865
ATTTCCA1398350.0105.5843667
TTTCCAC1399650.0105.216998
TTCCACA1471450.0100.063189
CGGCAAT33400.078.3955462
ACGGCAA40950.064.3107151
GTTCCAC88350.061.00215
GCAAGTT80400.054.200021
AGACCGG34150.053.044131
GACCGGA34650.052.2674672
GGCAATT54700.050.660913
ATGCAAT133050.047.7237662
Produced by FastQC (version 0.11.5)