FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4988459
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT54784510.982249227667301Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT220740.44250138168921505Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT198400.3977180127169533Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT158750.3182345489859694Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT144160.2889870398854636Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT120440.24143728554248917Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT116360.23325840705516473Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT70610.14154671813479874Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT64720.1297394646322642Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT61400.12308410272591196Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT255400.0123.534842
TGCAATT295250.0122.507083
GCAATTT368350.0114.5817954
ACTGCAA269200.0114.3327561
CGCAATT34550.0113.824883
CAATTTC422800.0111.989235
AATTTCC448450.0111.432026
TTTCCAC460900.0107.6738
ATTTCCA470250.0106.056247
CCGCAAT32450.0104.205832
GACCGGA40850.0102.4954452
TTCCACA487400.0101.9080359
AGACCGG41300.098.228891
ACCGCAA36600.089.824451
TGATCGG14150.088.216431
ACATCGG24900.085.1061551
ACCGGAA50650.083.94683
CGGCAAT18100.082.987482
CATCGGA28100.076.4374542
ACGGAAG42050.071.089994
Produced by FastQC (version 0.11.5)