FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8468236
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT103256912.193436744086963Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT89880.10613780721274184Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT89570.10577173333383717Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1065550.0134.070241
CTGCAAT1069550.0133.050162
CCGCAAT138300.0127.3014452
TGCAATT1231800.0126.9899753
ACCGCAA141800.0124.461181
CGCAATT151400.0123.2290343
GCAATTT1672950.0105.2454154
CAATTTC1698450.0103.840345
AATTTCC1706650.0103.7322166
TTTCCAC1703150.0103.239298
TTCCACA1727650.0102.1145559
ATTTCCA1739400.0101.562897
CGGCAAT24200.072.811132
ATGCAAT151650.057.330212
ACGGCAA31800.055.2476041
ATTGCAA80100.051.4802931
GGCAATT37950.049.6776053
TATGCAA56900.044.5285341
ACGCAAT19850.038.3562052
AATCGGA43950.038.2768252
Produced by FastQC (version 0.11.5)