FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13015300
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT11456388.802240440097425Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1336550.0132.209982
ACTGCAA1361450.0130.131
TGCAATT1525450.0126.108563
CCGCAAT190650.0125.127582
CGCAATT201150.0124.506273
ACCGCAA195100.0122.6294251
GCAATTT2016750.0108.589284
AATTTCC2063100.0106.6366356
CAATTTC2061050.0106.490985
TTTCCAC2071800.0105.320428
TTCCACA2102250.0103.860049
ATTTCCA2121250.0103.350557
CGGCAAT35350.071.791432
ACGGCAA46250.055.2453421
ATGCAAT203450.047.757442
ATTGCAA121100.045.7945631
GGCAATT61900.042.521143
TCCGATC55450.041.9751635
TTCCGAT58300.040.0475774
CCGATCT64050.035.430656
Produced by FastQC (version 0.11.5)