FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11493812
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT8356607.270520868098417Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT575110.5003648919957974Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT297720.25902633521411345Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT267800.23299493675379415Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT242260.2107742844584547Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT876850.0127.96242
TGCAATT983950.0124.64293
ACTGCAA908550.0124.166011
CGCAATT123100.0119.4900743
CCGCAAT118200.0118.166512
ACCGCAA121200.0115.394881
GCAATTT1244650.0112.0003364
AATTTCC1373550.0110.13216
CAATTTC1322500.0108.766775
TTTCCAC1414350.0106.259498
ATTTCCA1439950.0104.7378547
TTCCACA1489500.0100.947039
GACCGGA56750.086.270012
AGACCGG59450.082.253271
AGATTGG141000.074.315461
GATTGGA142600.073.358432
CGGCAAT34300.070.837462
ACCGGAA70000.069.524343
AGAACGG65800.067.901331
GAACGGA66000.066.5738452
Produced by FastQC (version 0.11.5)