FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10547150
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT7546367.154880702369834Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT305130.2893009011913171Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT238400.226032624927113Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT210140.1992386568883537Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT169930.16111461390043758Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT612150.0124.278132
TGCAATT654650.0123.196643
ACTGCAA635050.0118.5047451
CGCAATT75050.0113.867013
GCAATTT805250.0112.463154
AATTTCC911600.0109.992296
CAATTTC872950.0108.3220445
TTTCCAC945050.0105.3968058
ATTTCCA962300.0103.996967
CCGCAAT80450.0103.8779752
TTCCACA996250.0100.358359
ACCGCAA85200.096.836441
GACCGGA57900.082.387442
AGACCGG56800.082.21721
ACCGGAA69900.068.554963
TGATCGG19500.059.2192041
GATTGGA99350.058.0996442
CGGCAAT26950.057.653972
AGATTGG101600.055.9716531
GAACGGA62450.052.2021182
Produced by FastQC (version 0.11.5)