FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13584898
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT136306610.033685935661792Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT345400.25425292114817494Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT307470.2263322109595523Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT223020.16416759257228136Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT214060.15757203329756322Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATT683600.0123.115133
CTGCAAT649450.0122.2403262
ACTGCAA696900.0113.968791
GCAATTT854900.0110.012394
CGCAATT80100.0107.6163
AATTTCC930750.0106.8184366
CAATTTC915500.0105.345725
TTTCCAC972900.0101.838388
ATTTCCA999600.099.2777947
TTCCACA1011600.098.03569
CCGCAAT87050.095.165922
ACCGCAA96850.085.5234761
GACCGGA70850.075.3567352
AGACCGG77150.069.347511
ACCGGAA96150.055.919313
GATTGGA116500.052.1175082
AGATTGG117700.051.3152241
ACGGAAG75550.050.42084
GAACGGA76500.049.2141532
ATTGGAA127800.046.7831273
Produced by FastQC (version 0.11.5)