FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005483962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12078489
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT655092954.236328732840676Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2610852.1615700440675982Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT2368111.9606011977160387Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1869991.5481986198770392Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1725411.428498216954124Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1441411.193369468647941Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT1436401.1892215988274693Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT847900.7019917805944105Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT763140.6318174400788046Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT752120.6226937823100224Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT566620.46911496959594867Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC424020.35105384456615396RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC415400.34391719030418455RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC339720.2812603463893538Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC230690.19099243291110338RNA PCR Primer, Index 16 (100% over 22bp)
AGAGCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT219750.1819350085925483Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC201690.16698280720378186TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC182500.15109505833055772TruSeq Adapter, Index 16 (95% over 22bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT165270.13683002898789742Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC160740.13307955986878822TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC156120.12925457811817356Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC124060.10271152293966572Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)