FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6591453
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT328872949.89383979526213Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT829521.2584782141357906Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT741611.12510853069877Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT532720.8081981317321083Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT514000.7797977168311752Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC330260.5010427898067391Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT206340.31304175270611806Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT197490.29961527450776027Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGACGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT195040.2958983398652771Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTCTGCCGTCTT191890.2911194238963701Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC171360.2599730287085412RNA PCR Primer, Index 16 (96% over 25bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC170950.259351011074493RNA PCR Primer, Index 16 (96% over 25bp)
CTCTTCCGATCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTC133120.20195850596219073Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC131640.19971317401489475Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCG117750.17864043026628576Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGT112070.17002321036044707Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC88170.13376413364397804RNA PCR Primer, Index 16 (100% over 22bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC77860.11812266582193638TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC75580.11466364093015606TruSeq Adapter, Index 16 (95% over 22bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCCATCTCGTATGCCGTCTTC65960.10006898327273213TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT634200.0136.599012
ACTGCAA647650.0134.221481
TGCAATT705850.0132.31713
GATTGGA117150.0121.5453642
AGATTGG118050.0120.268921
GACCGGA108950.0116.778462
AGACCGG109000.0116.736311
CGCAATT86550.0115.2704543
CCGCAAT81850.0115.031392
TTGGAAG123100.0113.87324
ATTGGAA123750.0113.512193
GCAATTT941000.0111.4167944
AATTTCC997750.0111.084576
CAATTTC972950.0110.614225
ATTTCCA1008300.0109.615217
TTTCCAC1012100.0108.887588
ACCGGAA118050.0107.622363
CCGGAAG119950.0106.3386464
TTCCACA1063500.0103.747679
AGATGGG97150.0100.491671
Produced by FastQC (version 0.11.5)