FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9801625
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT134263613.698095978983076Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT453190.46236210832387487Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT273790.27933123334141025Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT262750.26806779488095084Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT233120.23783811357810566Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT624850.0128.992872
TGCAATT707150.0123.934693
ACTGCAA659050.0122.659971
CGCAATT87100.0110.9649353
GCAATTT910200.0108.264894
CCGCAAT83750.0107.764032
AATTTCC983850.0105.848936
CAATTTC958700.0105.019185
TTTCCAC1005300.0103.004618
ATTTCCA1025200.0101.409267
ACCGCAA90000.0100.835381
TTCCACA1055550.098.35319
GACCGGA54150.084.8140642
AGACCGG54900.084.3766251
GATTGGA105350.075.582242
AGATTGG109300.073.1677251
ACCGGAA67700.067.0813453
GAACGGA62700.063.729682
ATTGGAA124550.063.4588133
AGAACGG63650.063.0507931
Produced by FastQC (version 0.11.5)