FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12674311
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT5176854.08452183317894Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT1196000.0130.671952
ACTGCAA1204950.0130.254611
CGCAATT170700.0125.182083
TGCAATT1382400.0124.7412643
ACCGCAA162500.0123.121041
CCGCAAT163700.0122.1222
GCAATTT1817450.0107.369454
AATTTCC1864450.0105.211086
CAATTTC1861650.0104.9995
TTTCCAC1874050.0103.737468
TTCCACA1903600.0102.263829
ATTTCCA1921350.0101.711927
CGGCAAT34000.065.686732
ACGGCAA41100.053.8721731
ATGCAAT184800.050.6559072
ATTGCAA110250.046.684651
TATGCAA83250.038.542911
GGCAATT59900.038.4942473
ACGCAAT25950.037.16382
TCCGATC43300.034.8314065
Produced by FastQC (version 0.11.5)