FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8614980
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT6211797.210452026586249Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ACTGCAATTTCCACAGGGGTAGTCGATCCGATGTTTACTCGGATGGAAAA91440.1061406991078331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAA1144000.0134.153961
CTGCAAT1151200.0132.871282
TGCAATT1366050.0127.1894763
ACCGCAA161800.0125.6839141
CCGCAAT161100.0125.673242
CGCAATT178550.0123.17583
GCAATTT1858000.0106.1662454
AATTTCC1885950.0104.891926
CAATTTC1884800.0104.729765
TTTCCAC1880900.0104.245788
ATTTCCA1912650.0103.004297
TTCCACA1906650.0102.935579
CGGCAAT27550.077.383552
ATGCAAT185450.071.399782
ACGGCAA33150.064.169581
TATGCAA60850.063.8319781
ATTGCAA78250.059.565641
CATGCAA69750.055.79121
GGCAATT40900.055.315153
ACGCAAT24050.049.146822
Produced by FastQC (version 0.11.5)