FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005483998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005483998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10264634
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT7712767.513916229258637Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATTGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT544850.5308031440770319Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGATGGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT279410.2722064907526172Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGACCGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT255440.2488544647573406Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
AGAACGGAAGAGCACACGTCTGAACTCCAGTCCATCTCGTATGCCGTCTT228790.22289153222608812Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCAAT972250.0133.127532
ACTGCAA1000400.0129.786581
TGCAATT1100100.0128.99443
CGCAATT134050.0123.990223
CCGCAAT128300.0121.951192
ACCGCAA132100.0118.915031
GCAATTT1430000.0112.5105444
AATTTCC1562800.0111.746216
CAATTTC1499250.0110.945435
ATTTCCA1606750.0108.3512347
TTTCCAC1612250.0107.563488
TTCCACA1700300.0102.155319
GACCGGA53200.082.857782
AGACCGG53300.081.768591
AGATTGG120150.078.96611
GATTGGA120850.078.0666662
CGGCAAT35700.075.796122
GTTCCAC98150.070.55085
ATTGGAA134200.069.920613
ACGGAAG59350.065.9364244
Produced by FastQC (version 0.11.5)