FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005484129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005484129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46966068
Sequences flagged as poor quality0
Sequence length150
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2269190.48315520047366967No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG975060.20760945966351707Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)