FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005484435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005484435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46464229
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3303980.7110803452694759TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2375780.5113137678449372TruSeq Adapter, Index 18 (97% over 36bp)
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC1681150.3618159681504669No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT942900.20293030150139799No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT810240.17437930585268077No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA728430.15677221287799697TruSeq Adapter, Index 18 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA640370.13781999912233558No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG578860.12458185844426689No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG569210.12250499195843755No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG542940.11685118029183268No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT508100.1093529390103514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)