FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005484445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005484445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52609956
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC4234140.8048172478988578No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1191940.22656167969423885No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1023330.19451261278378562No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1016790.19326950206915208No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG674160.12814304577635457No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC663550.1261263172316662No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG663120.1260445836525695No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT630870.11991456522031686No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG597930.11365339290532765No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA591330.11239887750523875TruSeq Adapter, Index 11 (97% over 36bp)
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG536080.10189706298176719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)