FastQCFastQC Report
Thu 12 May 2022
EGAF00005484447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005484447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47271062
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3310220.7002635142827973TruSeq Adapter, Index 18 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2352800.4977252256359292TruSeq Adapter, Index 18 (97% over 36bp)
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC1741460.36839874678508383No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT939500.19874738587425853No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT807020.17072178323389475No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA716850.15164668820006624TruSeq Adapter, Index 18 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA640960.135592468813161No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG578890.12246181395289997No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG575560.12175736606044518No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG555690.11755394875621791No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT502910.10638855543376623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)