FastQCFastQC Report
Thu 12 May 2022
EGAF00005484448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005484448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47271062
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGCACCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA2764770.5848757956823564Illumina Single End PCR Primer 1 (96% over 33bp)
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCG681470.14416219377512612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)