FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18799892
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13450.085.158459
TTTGCGC28600.080.451941
CGCCGAA29500.078.991081
TGCGCGA27150.078.810841
ACGACCG27400.073.010451
GACGCGT27650.070.4951861
GTGCGAC32800.069.703361
TTACCCG36800.069.4945141
GCGCTAG32850.069.374191
CGGTACG30150.068.538531
TCACGCG27450.067.606838
GCGCGTA28150.067.1605451
ATAACGC35550.065.548151
CGAACGC35800.065.499791
TCCGCGA29050.065.380488
GGCGACT38700.064.946551
CCGCTAT33700.064.145331
TCGTACG34350.062.8901258
GTTACGC31600.062.85748
CCTTACG36200.062.751781
Produced by FastQC (version 0.11.5)