FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18900466
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11650.084.00589
TTTGCGC30450.079.090211
ACGACCG26550.074.992631
TGCGCGA28650.072.5617751
TTACCCG36800.071.211381
GTGCGAC30900.071.068471
TCACGCG26650.070.18178
CGGTACG28800.069.6421661
CGCCGAA30250.068.723791
ATAACGC37050.066.779421
GCGCGTA31500.066.69381
GACGCGT27750.065.682631
GCGCTAG31250.065.587661
TCCGCGA28900.065.219418
GGCGACT36650.064.512331
CGAACGC36000.064.457121
AACGCGT24100.063.7695433
TCGTACG34200.062.955228
GTTACGC29850.062.6580358
GTACGCT27550.062.1000338
Produced by FastQC (version 0.11.5)