FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20123910
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA31350.079.977411
CGCCGAA29050.076.244271
TTACGCG11600.074.991629
GTGCGAC33750.074.506711
TTTGCGC31950.072.755691
CGGTACG31900.072.640591
ATAACGC36100.069.859041
ACGACCG26800.068.4620061
TTACCCG40600.066.0770651
TAGGCGC36600.065.5093841
GACGCGT33450.064.685411
GCGCTAG36050.064.4811251
GCGCGTA34600.064.4368741
CGTTGCG16250.064.239137
AACGCGT28150.064.12253
GTACGCT27750.064.0018548
CGAACTA43750.062.9903531
CCGCTAT37850.061.994031
CGAACGC37100.061.671021
GGCGACT40900.061.302971
Produced by FastQC (version 0.11.5)