FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20186698
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30400.086.071121
TTACGCG13000.084.762099
CGCCGAA32700.078.224081
TTTGCGC30750.078.179251
CGGTACG29600.075.273951
GCGCTAG34200.071.79291
ACGACCG31250.070.126791
GACGCGT31300.069.312281
GTGCGAC34150.067.820221
TAGGCGC37950.067.59571
TTACCCG41150.066.079521
GCGCGTA33350.065.930781
CCGCTAT38600.065.318161
ATAACGC35750.065.194941
CCTTACG41150.065.188961
GTTACGC32300.064.862968
TCGTTCG35100.064.645558
GCCGGAT38850.064.143211
GTTCGCG38700.063.6897288
CGCGGTA39650.062.8490261
Produced by FastQC (version 0.11.5)