FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16700444
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25950.0102.2395866
ACGACGC27600.098.228595
CACGACG28750.093.7951664
GACGCTC37400.070.357717
TACACGA41650.066.570122
CTACACG44400.062.2950481
ACGCTCT43950.059.8719148
ACACGAC45800.058.8779683
CGCTCTT57250.045.837559
TCTTCCG77750.07.66411810-14
CTTCCGA83950.07.581663610-14
CGTAAAG22305.5479177E-107.56690171
TTCCGAT99950.06.745138610-14
CGATCTA27200.06.50302715-19
TCCGATC101400.06.14830115-19
CCGATCT110450.05.99894815-19
AACGGGG29753.4302502E-85.91860531
GCAATCG25553.4653258E-65.6743296
TAACCCT580000.05.58669426
AACGGAG36903.2123353E-95.56707141
Produced by FastQC (version 0.11.5)