FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17969619
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10350.081.248579
TTTGCGC30500.079.304631
CGCCGAA26150.078.2018361
TGCGCGA25750.076.0008541
ACGACCG28000.075.6526641
CGGTACG30050.071.223411
GTGCGAC32550.071.157471
GCGCGTA27650.069.187841
GGCGACT35350.068.0093841
ATAACGC35200.066.21691
GTACGCT26300.066.153818
CGAACGC32600.066.101941
TTACCCG35150.065.685521
GACGCGT26750.065.213521
GCGCTAG29200.065.013171
CGCGGTA34350.064.441351
GTTCGCG34100.062.0765848
AACGCGT25800.061.8158533
TCGTACG34150.061.7734158
ACGCCGA34450.061.701141
Produced by FastQC (version 0.11.5)