FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17857677
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26750.081.8264851
CGCCGAA25250.080.309121
TTACGCG10500.078.7069859
ACGACCG26600.074.306811
CGGTACG29600.074.195241
GTGCGAC30200.073.690771
GCGCTAG32850.070.19751
TTTGCGC26000.070.1087951
GCGCGTA28100.070.0797351
ATAACGC32350.069.9247061
GACGCGT28000.069.0227661
TTACCCG33150.068.67891
GGCGACT35100.068.617561
CGAACGC34400.067.460151
CCGCTAT34100.067.194921
TAGGCGC31400.065.046071
AACCGCG35050.064.3293461
CGCGGTA35900.063.4179841
AACGCGT26050.062.891913
ACGCCGA33700.062.5617751
Produced by FastQC (version 0.11.5)