FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16663272
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT28000.0107.185796
ACGACGC29600.0102.138035
CACGACG31000.097.993094
GACGCTC40400.074.4670647
TACACGA45100.068.65572
CTACACG46600.065.083561
ACGCTCT48100.063.2993358
ACACGAC49800.061.436653
CGCTCTT57600.052.2300579
TCTTCCG83700.08.228085510-14
CTTCCGA91000.07.95041510-14
TTCCGAT112850.06.66801310-14
TCCGATC112600.06.59281815-19
CGATCTA29300.06.2845715-19
CCGATCT122650.06.277207415-19
CGAAGTC21557.879591E-66.05577373
CGTTGAG23003.2167009E-66.02267741
AACGGGG30501.0571966E-85.97590971
CGCTGAT25051.3603914E-55.52980381
TAACCCT588350.05.5199756
Produced by FastQC (version 0.11.5)