FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19686072
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12450.083.846089
TTTGCGC30300.082.2737351
TGCGCGA31150.078.385891
GTGCGAC36300.075.119331
GCGCTAG33950.073.4284061
ACGACCG28600.072.8497851
CGGTACG31150.071.814611
CGCCGAA32400.070.39781
GACGCGT31650.069.063231
TCGTACG39650.067.8297658
CCGCTAT39250.067.238341
GCGCGTA32900.066.883671
CCTTACG37050.065.903381
TTACCCG36400.065.674361
CGAACGC34850.064.190111
GTACCGT48050.063.364438
CGTTGCG15650.062.533447
CGAACTA42400.062.415461
ACGCCGA38500.062.282011
ATAACGC35100.061.8584181
Produced by FastQC (version 0.11.5)