FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20049893
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13450.086.237259
TGCGCGA30350.077.64281
CGCCGAA30450.076.907141
TTTGCGC30700.076.519231
GTGCGAC34200.074.679831
CGGTACG33050.070.192671
ACGACCG27750.069.621751
GCGCTAG35000.068.1637651
GCGCGTA35200.067.5685651
GGCGACT38000.066.8266751
CGAACGC37850.066.704821
AACGCGT26750.066.12433
GACGCGT32450.065.852481
TAGGCGC36800.065.6251141
TTCCGCG18000.064.841137
GCCGGAT42400.064.72461
GTTACGC31950.064.438398
TTACCCG39050.064.2801741
ATAACGC38400.064.034211
CCGCTAT39700.063.9650841
Produced by FastQC (version 0.11.5)