FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21445125
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC30700.082.654231
TTACGCG12350.081.5913249
TGCGCGA32200.078.576121
CGCCGAA36800.073.138421
ACGACCG32600.071.538021
GCGCTAG35450.070.544881
CGAACGC39850.067.540631
GGCGACT42550.066.701981
CGGTACG32850.066.528571
TAGGCGC39450.065.622841
GTGCGAC36250.065.548741
GTTACGC32900.065.2224358
GACGCGT37350.064.207311
CGAACTA47150.064.0831
GTACGCT31600.064.005658
CCGCTAT42850.063.1542931
CGATCGG40300.062.600921
TTACCCG43350.062.256691
CGACACG43000.062.2517781
CTTACCG48050.061.9669271
Produced by FastQC (version 0.11.5)