FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21399683
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13500.083.231219
TTTGCGC29700.079.508781
TGCGCGA33000.077.3179861
CGCCGAA35800.075.96771
CGGTACG33500.072.235751
ACGACCG31750.071.842251
GTGCGAC33700.069.420721
TCGTACG40550.067.933048
TCACGCG28250.066.974638
TTACCCG40750.066.022091
GCGCTAG37000.065.9954151
GTTACGC33300.064.654768
CGAACTA45800.064.648521
CGAACGC40900.063.45621
GCCGGAT41200.062.994141
GCGCGTA36800.062.976781
GGCGACT40050.062.2473531
GACGCGT33750.061.736231
AACCGCG38600.061.3658521
CCGAACG22450.060.3828439
Produced by FastQC (version 0.11.5)